NOS2 anticorps (AA 40-1099)

N° du produit ABIN7601635

L’anticorps Lapin Polyclonal anti-NOS2 a été validé pour WB, ELISA et FACS. Il convient pour détecter NOS2 dans des échantillons de Souris.

Aperçu rapide pour NOS2 anticorps (AA 40-1099) (ABIN7601635)

Antigène: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NOS2 est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Détail du produit anti-NOS2 anticorps

Épitope: AA 40-1099

Fonction: Anti-iNOS/Nos2 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-iNOS/Nos2 Antibody Picoband® (ABIN7601635). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived mouse iNOS/Nos2 recombinant protein (Position: I40-L1099).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Chartrain, N. A., Geller, D. A., Koty, P. P., Sitrin, N. F., Nussler, A. K., Hoffman, E. P., Billiar, T. R., Hutchinson, N. I., Mudgett, J. S. Molecular cloning, structure, and chromosomal localization of the human inducible nitric oxide synthase gene. J. Biol. Chem. 269: 6765-6772, 1994. 2. Diefenbach, A., Schindler, H., Rollinghoff, M., Yokoyama, W. M., Bogdan, C. Requirement for type 2 NO synthase for IL-12 signaling in innate immunity. Science 284: 951-955, 1999. 3. Hobbs, M. R., Udhayakumar, V., Levesque, M. C., Booth, J., Roberts, J. M., Tkachuk, A. N., Pole, A., Coon, H., Kariuki, S., Nahlen, B. L., Mwaikambo, E. D., Lai, A. L., Granger, D. L., Anstey, N. M., Weinberg, J. B. A new NOS2 promoter polymorphism associated with increased nitric oxide production and protection from severe malaria in Tanzanian and Kenyan children. Lancet 360: 1468-1475, 2002.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur NOS2

Antigène: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Autre désignation: Nos2

Sujet:

Synonyms: Nitric oxide synthase, inducible,1.14.13.39,Hepatocyte NOS,HEP-NOS,Inducible NO synthase,Inducible NOS,iNOS,N

Tissue Specificity: Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.

Background: Nitric oxide synthase, inducible is an enzyme that in humans is encoded by the NOS2 gene. Nitric oxide (NO) is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter, it is implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. Three different NOS isoforms have been identified which fall into two distinct types, constitutive and inducible. The inducible NOS (iNOS) isoform is expressed in a variety of cell types and tissues in response to inflammatory agents and cytokines. The human iNOS (NOS2) gene is approximately 37 kb in length and consists of 26 exons and 25 introns. NOS2-derived NO is a prerequisite for cytokine signaling and function in innate immunity.

Poids moléculaire: 130 kDa

ID gène: 18126

UniProt: P29477

Pathways: Retinoic Acid Receptor Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Inositol Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process